The study adapted the Perceived Accessibility Scale to assess perceived cycling accessibility in Stockholm, presenting a new case study of its application. Using recent survey data and factor analysis, the scale demonstrated strong reliability in capturing perceptions. Hypothesis testing indicated limited effects of most sociodemographic factors on perceived cycling accessibility, except for gender among frequent cyclists. Mobility-related characteristics and peer influence had stronger effects, while spatial variables were insignificant. A significant positive association was also found between perceived cycling accessibility and cycling frequency. These findings align with expectations and reflect behaviour of Swedish cyclists.