MicroCap: Microfluidic centrifuge assisted precipitation for DNA quantification on lab-on-DVDShow others and affiliations
2018 (English)In: 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Chemical and Biological Microsystems Society , 2018, p. 1802-1805Conference paper, Published paper (Refereed)
Abstract [en]
We report for the first time the MicroCAP technique, for rapid DNA detection and quantification, that does not require any purification or fluorescent labelling of DNA. The invention is based on DNA interacting with a detection dye (Gelred) to form a complex, that forms a visible precipitate within seconds of centrifugation. MicroCAP can be used for DNA quantification, when combined with the Lab-on-DVD with inbuilt centrifugation and sub-micron imaging resolution. We quantify PCR and LAMP assay products using MicroCAP on the integrated Lab-on-DVD platform, and demonstrate a detection limit of 10 ng/μl. Copyright
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2018. p. 1802-1805
Keywords [en]
Centrifuge, DNA detection, LAMP, MicroCAP, PCR, Precipitate, Centrifugation, Centrifuges, Data storage equipment, DNA, Electric lamps, Precipitates, Videodisks, Detection limits, DNA quantification, Imaging resolutions, Sub micron, Polymerase chain reaction
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-276505Scopus ID: 2-s2.0-85079758085ISBN: 9781510897571 (print)OAI: oai:DiVA.org:kth-276505DiVA, id: diva2:1442380
Conference
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, 11 November 2018 through 15 November 2018
Note
QC 20200617
2020-06-172020-06-172022-06-26Bibliographically approved