Droplet based directed evolution of yeast cell factories doubles production of industrial enzymesShow others and affiliations
2013 (English)In: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Chemical and Biological Microsystems Society , 2013, p. 1270-1272Conference paper, Published paper (Refereed)
Abstract [en]
We present a high throughput micro-droplet based method for directed evolution of yeast cell factories for improved production of industrial enzymes. The workflow includes a fluorescently activated droplet sorter which was found to accurately sort droplets with a false positive rate of 0.0002 at 300 Hz. The workflow was used to screen a library of α-amylase expressing yeast mutants. A candidate yeast strain with a more than twofold increase in α-amylase production was isolated from a single round of directed evolution.
Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2013. p. 1270-1272
Keywords [en]
Amylases, Enzymes, Throughput, Yeast, Amylase production, Directed evolution, Droplet microfluidics, False positive rates, High throughput, Industrial enzymes, Micro-droplet, Yeast strain, Drops
National Category
Biochemistry Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-280428Scopus ID: 2-s2.0-84892513319ISBN: 9781632666246 (print)OAI: oai:DiVA.org:kth-280428DiVA, id: diva2:1469304
Conference
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 27 October 2013 through 31 October 2013, Freiburg
Note
QC 20200921
2020-09-212020-09-212025-02-20Bibliographically approved