In this work, the compressibility of Pickering-stabilized perfluoropentane droplets was determined by using acoustophoresis. Polyamide beads with known density, size and compressibility were used to calculate the pressure amplitude inside the microchannel. The results show that the compressibility of CNF-stabilized droplets is significantly higher than for water, but lower than for pure PFC5. This shows promising potential for these droplets to be used in ultrasound-mediated clinical applications. It has also been shown that acoustophoresis can successfully measure the compressibility of pressure-sensitive particles for small USW pressure amplitudes. As the droplets relocate to pressure anti-nodes just as gas-filled microbubbles, it would be possible to study cell-droplet and cell-gasbubble in the same  setup.