Solving many current societal challenges requires collaboration between numerous actors from different disciplines. However, there are several challenges in these interdisciplinary collaborations that have managerial implications, which this article aims to describe. The study is based on observations and fourteen interviews with actors from three innovation ecosystems (IES) that attack the challenges with the help of digitalization and enabling technology. Examples of identified challenges are difficulties for participating competitors to cooperate, that actors may feel threatened by the approaching result from the collaboration, that the actors may have different underlying motives for participating in the IES, and difficulties for actors to understand each other due to different domain knowledge.