This study investigated the effects of load case (varying extra load in addition to a single passenger) and tyre pressure (50, 200, 400 kPa) on the whole-body vibration of children being transported in a cycle carrier. The experiments were performed on a special test bed designed for this purpose. Since previous work indicated non-linear influence of the tyres, static and dynamic tyre stiffness were measured separately. Overall, vibration total values were in a similar level as the results of previous on road experiments. The tyres seemed to have some eigenfrequency that was rather independent of the vertical load but affected the pattern of the vibration experienced by the carrier passengers.