The automotive industry is heading towards a more objective approach to vehicle testing, but subjective evaluation is still an important part of the development process. Subjective evaluation in physical testing has environmental implications and is dependent on ambient conditions. A more repeatable, faster, safer and more cost-effective tool for subjective evaluation is to use moving base driving simulators. The motion cueing algorithms (MCA) maps the movement of the vehicle into the limited space of the simulator. The choice of reference point, that is, where on the vehicle to sample the motion to feed to the MCA and the alignment of the axis of rotation of the simulator cabin is still an open topic. This paper investigates the choice of reference point and corresponding simulator longitudinal axis of rotation in roll using two methods. The first method uses a linearised model of the combined system of vehicle, simulator and vestibular models. The second method, to position the cabin longitudinal axis of rotation, is based on offline optimisation. The linear model can capture important characteristics of the specific forces and rotations that are fed to the driver through the motion cueing algorithms and offers a method to objectively analyse and potentially tune the motion cueing. The analysis is further complemented with a subjective evaluation of corresponding settings. The results from the linear model, the offline optimisation and the subjective evaluation shows that a reference point at the driver’s head has a clear advantage over the full frequency range compared to a reference point in the chassis roll axis and that the positioning of the cabin longitudinal axis of rotation has a significant effect on the perceived vehicle characteristics.