Thermal depolymerization of lignocellulosic biomass at 400-600 oC in the absence of oxygen yields liquid, char, and gas. The presence of high oxygenates (40 wt.%) in the liquid fraction contributes to deleterious properties such as low heating value, high viscosity, corrosiveness, and low thermal stability. The present study investigates an upgrading strategy for crude biogenic pyrolysis oil using various adsorbents (calcium hydroxide, red mud, bentonite, dolomite, used silica, and new silica) with petroleum ether as a diluent. The textural and microstructural features of the adsorbents are analysed using various characterization techniques - a scanning electron microscopy (SEM) and a Brunauer–Emmett–Teller (BET) specific surface area analyser. The objective of the study was to induce a separation of soluble and insoluble phases in petroleum ether (upper homogeneous fraction and lower non-homogeneous fraction with adsorbents) using various cheap adsorbents, and to find the best adsorbent for the refining process. The original crude oil and the refined oil from the upgradation strategy were extensively characterized using multiple analytical techniques to understand the chemical, thermal, and stability characteristics. The results showed that calcium hydroxide is more effective in the removal of oxygenates in comparison with other adsorbents due to variations in surface area.