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Alternative Forward Models for Imaging Thick Specimens in Transmission Electron Microscopy
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems, Structural Biotechnology.ORCID iD: 0000-0002-0569-3374
2023 (English)In: Microscopy and Microanalysis, ISSN 1431-9276, E-ISSN 1435-8115, Vol. 29, no 3, p. 1071-1076Article in journal (Refereed) Published
Abstract [en]

I have investigated two different forward models for image formation in transmission electron microscopy of thick specimens, the 3DCtf model, which introduces a defocus gradient in the linear approximation, and the multislice model. An important result is that the 3DCtf model does not seem to be compatible with the multislice image formation model. A second very useful finding is that the exit wave in the multislice model has an imaginary part, which, in first-order approximation, is a pure projection of the specimen and is not affected by the defocus gradient. The defocus gradient only comes into play in real valued and higher-order imaginary terms. If the multislice model is closer to reality than the 3DCtf-model, then the best way to retrieve the specimen projection for thicker specimens should be a procedure for retrieving the exit wave's imaginary term, for example using images recorded at different defocus values.

Place, publisher, year, edition, pages
Oxford University Press (OUP) , 2023. Vol. 29, no 3, p. 1071-1076
Keywords [en]
3DCtf, defocus gradient, multislice, phase objects, TEM
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-336573DOI: 10.1093/micmic/ozad038ISI: 000972979800001PubMedID: 37749689Scopus ID: 2-s2.0-85168726792OAI: oai:DiVA.org:kth-336573DiVA, id: diva2:1797972
Note

QC 20230918

Available from: 2023-09-18 Created: 2023-09-18 Last updated: 2024-08-28Bibliographically approved

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Koeck, Philip J. B.

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