The KTH Library has a long tradition of teaching information searching to technology students. Over the last years teaching information searching has become teaching information literacy, including more evaluation and source criticism. Traditionally, there have been three forms of teaching: independent credit-giving courses, integrated shorter modules in subject-specific courses and support via individual face-to-face supervision.

Although evaluation and source criticism are now important parts of our teaching, much focus is still on search methodology. During this spring we, and our teaching colleagues at the KTH library, will revise and develop the content and pedagogical methodology for the courses and integrated modules in information literacy. In doing so, we need to address important questions on how to face the changing information landscape.

Should the teaching be adapted to the search behaviour observed in our students or should we keep trying to change that behaviour? Do we put our effort into directing students to traditional scientific subject databases or should we put more emphasis on the importance of critically evaluating the search results, regardless of their source? How do we find the balance between these alternatives?

Recently published studies have already covered these questions to some extent. The findings from a systematic literature search, together with insights collected from our development work during Spring 2016, will be used in an analysis of these questions in the context of teaching information literacy for engineering students.

LysR Type Transcriptional Regulators (LTTRs) regulate basic metabolic pathways or virulence gene expression in prokaryotes. Evidence suggests that the activation of LTTRs involves a conformational change from an inactive compact apo-configuration that represses transcription to an active, expanded holo-form that promotes it. However, no LTTR has yet been observed to adopt both configurations. Here, we report the results of structural studies of various forms of the LTTR DntR. Crystal structures of apo-DntR and of a partially autoinducing mutant H169T-DntR suggest that active and inactive DntR maintain a compact homotetrameric configuration. However, Small Angle X-ray Scattering (SAXS) studies on solutions of apo-, H169T- and inducer-bound holo-DntR indicate a different behaviour, suggesting that while apo- DntR maintains a compact configuration in solution both H169T- and holo-DntR adopt an expanded conformation. Models of the SAXS-obtained solution conformations of apo- and holo-DntR homotetramers in complex with promoter-operator region DNA are consistent with previous observations of a shifting of LTTR DNA binding sites upon activation and a consequent relaxation in the bend of the promoter-operator region DNA. Our results thus provide clear evidence at the molecular level which strongly supports the 'sliding dimer' hypothesis concerning LTTR activation mechanisms.